Quantitative Assessment of Extracellular Matrix Effect on A549 Attachment and Spreading
The Cell Index increases proportionately as the coating concentration of collagen IV increases. (Data and figures adapted from ACEA Biosciences, unpublished data).
Mammary Stem-Like Cells Have Increased Tubulin-Dependent Initial Reattachment From Suspension
(A) Stem-like HMLE cells attach at signifi cantly faster rates than non-stem-like HMLE cells as determined by electrical impedance expressed as Cell Index. Stem-like HMLE and non-stem-like cells both have significantly reduced attachment when treated with the microtubule polymerization inhibitor colchicine (50µM). For all reattachment assay: lines, mean for quadruplicate wells; bars, SD; representative graph from three independent experiments is shown. (B) Phase contrast images of HMLE non-stem-like (a-d) and stem-like (e-h) subpopulations reattaching from suspension. Panels, 10x magnification; Insets, 60x magnification (Data and figures adapted from Charpentier MS, et al., 2014).
- Real-time monitoring of cell adhesion and spreading.
- Label-free assay requires no fixation, staining or any other sample processing.
- Direct, sensitive, and quantitative.
- Easy quantification of the kinetics of adhesion and spreading.
- Rapid optimization of cell density and extracellular matrix coating conditions.
Cell Adhesion/Spreading Supporting Information:
- Dynamic Monitoring of Cell Adhesion and Spreading
- Dynamic Monitoring of Cell Adhesion and Spreading Using iCELLigence
- Research Presentation Video:
- Targeting the Reattachment of Circulating Breast Tumor Stem Cells to Reduce Metastasis
Presented by Dr. Stuart Martin (University of Maryland School of Medicine, Baltimore, MD)
- Identifying a Novel Diagnostic and Therapeutic Target for Metastatic Breast Cancer
Presented by Dr. Michele I. Vitolo (University of Maryland, Baltimore, MD)
- Cell Adhesion/Spreading – Compatible xCELLigence System:
- Cell Adhesion and Spreading Publications
- Using cell-substrate impedance and live cell imaging to measure real-time changes in cellular adhesion and de-adhesion induced by matrix modification. Rees, M. D., Thomas, S. R. Vis. Exp. (96), e52423, doi:10.3791/52423 (2015).
- Curcumin targets breast cancer stem-like cells with microtentacles that persist in mammospheres and promote reattachment. Charpentier MS, Whipple RA, Vitolo MI, Boggs AE, Slovic J, Thompson KN, Bhandary L, Martin SS. Cancer Res. 2014 Feb 15;74(4):1250-60.
- New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells,highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma. Tibaldi L, Leyman S, Nicolas A, Notebaert S, Dewulf M, Ngo TH, Zuany-Amorim C,Amzallag N, Bernard-Pierrot I, Sastre-Garau X, Théry C. PLoS One. 2013 Aug 16;8(8):e72708.
- A role for adhesion and degranulation-promoting adapter protein in collagen-induced platelet activation mediated via integrin a2b1. Jarvis GE, Bihan D, Hamaia S, Pugh N, Ghevaert CJ, Pearce AC, Hughes CE, Watson SP,Ware J, Rudd CE, Farndale RW. J Thromb Haemost. 2012 Feb;10(2):268-77.
- Dynamic monitoring of cell adhesion and spreading on microelectronic sensor arrays. Josephine M. Atienza, Jenny Zhu, Xiaobo Wang, Xiao Xu and Yama Abassi. J Biomol Screen. 2005 Dec;10(8):795-805.
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