NovoCyte Flow Cytometer

RUONovoSampler

Address the full range of current and future multi-parameter analysis research needs with the NovoCyte flow cytometer. ACEA brings researchers high performance flow cytometry at a low investment cost with the NovoCyte platform.

ACEA offers a system which is:

  • Powerful- up to 15 parameter detection with enhanced sensitivity and resolution.
  • Intuitive – automated instrument maintenance functions and advanced data analysis capabilities for greater usability.
  • Customizable – 1 to 3 laser options, exchangeable filters, multiple sampling options, and flexible analysis formats.

Other important features enhance usability, such as fixed optical alignment, 24-bit detection dynamic ranges with no need for PMT voltage adjustment, accurate volumetric-based cell counting, pressure sensors to monitor fluidic status in real-time, and automatic cleaning and decontamination processes.
ACEA also developed the NovoExpress software, which is intuitive and easy to use. Flexible analysis templates and plotting tools offer enhanced data analysis efficiency.

NovoCyte Technical SpecificationsNovoCyte_Specifications_080116_025415_PM


Optimized PMT Voltage & 7 Decades Detection Dynamic Range

Provides 107 dynamic range for signal detection and processing, offering a broader signal range than other flow cytometers. Wide range of fluorescent and scattering signal intensities eliminates the need for complicated and laborious PMT voltage adjustment. Data acquisition is just load-and-go.

Configurable Fluorescence Detection Channels for Enhanced Assay Flexibility

Customizable selection and upgrade with lasers of different wavelengths allow for personalized, versatile choices of fluorochromes for flow cytometry assays. User interchangeable filters and dichroic mirrors broaden available fluorescence channels to expand users’ detection options.

13 Fluorescent Channel Detection: Multiple Laser Options Providing Flexibility of Choice (405nm, 488nm, 561nm, 640nm)

Customize the instrument to meet detection needs with blue, red and violet laser options. System can be upgraded as analytical requirements increase – no need to purchase another instrument. State-of-the-art solid-state lasers provide high quality and extremely stable optical illumination.

Novel Optical Design & Enhanced Signal Detection

Patent pending independent beam shaping optics and light collection system maximizes signal detection efficiency, increasing sensitivity and resolution for each fluorescence channel. Fixed optical alignment removes need for daily maintenance and adjustment of optical system, providing convenient operation and superior long-term stability. Optimized forward scatter obscuration bar increases sensitivity. Enhanced small particle detection and resolution as a result of optimized obscuration bar design.

Volumetric Fluidics System for Increased Accuracy & Easy Maintenance

The NovoCyte flow cytometer uses a highly accurate, volumetric syringe pump which enables direct absolute counting of samples, eliminating the need to use expensive reference beads.
volumetric-count
Real-time monitoring of fluidics prevents flow path obstruction. Accurate pressure sensors monitor fluidic status in real-time, thus the possibility of flow path obstruction is greatly reduced, allowing for reliable and accurate data acquisition. Automated SIP (sample injection probe) washing following sample acquisition minimizes sample carryover and increases capability to detect rare events. High speed data acquisition of 35,000 events per second.
QC
Routine maintenance features are as simple as clicking a button and following the on screen instructions

High Sensitivity & High Resolution Detection

Highly efficient light collection ensures reliable detection of sub-micron particles and dim signals.

Using innovative optical design for light collection and high quality PMTs, fluorescence signals are efficiently detected with exceptionally high signal-to-noise ratio, ensuring a high detection sensitivity for weak positive samples and small particles.
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High Fluorescent Signal Resolution Improves Detection Accuracy

Advanced optical and fluidic system design, premier quality components, and innovative signal processing algorithms collectively ensure accurate extraction of detection parameters with consistently low variation.
Spherotech8

Option for High-Throughput and Automated Analysis of Multiple Samples

The NovoSampler is a modular autosampler, that can be interchanged with the single tube loader in just a couple minutes, is compatible with 12 x 75mm tube, 1.5 & 2mL tubes and 24/48/96 well plates, allowing for versatile loading modes, and increased throughput. Automatic calibration is just the push of a button and this procedure ensures accuracy between different plate types. Simply select your plate, identify your wells or tubes to run, and start running. The NovoSampler will automatically switch between samples, employ mixing or rinsing steps between samples if desired, and complete your run all while you are away.


Experiment Analysis Design

Experiment Manager provides a schematic view of the sample analysis being performed.This allows for access to cytometer setting,compensation matrix, report generation, and data analysis. Multiple samples can be analyzed with the same settings by a simple drag-and-drop template function.

Automated & Versatile Data Analysis Functions

Powerful NovoExpress software allows efficient data acquisition, data analysis and report generation. NovoExpress software provides flexible analysis templates and plotting tools, offering enhanced data analysis efficiency.

NovoExpress Download

Download NovoExpress Software Now!

DataAnalysis

Pre & Post Acquisition Compensation

Instantaneous compensation results can be simply achieved by adjustment of the compensation scaling bar on the plot, allowing rapid and accurate compensation, thus eliminating tedious trial and error adjustments of compensation matrix coefficients.
A simple click of a button initiates the automated cleaning and de-contamination process and effectively removes any residual samples in the fluidics path, saving users’ valuable time from laborious manual cleaning and decontamination routines.
QC
QC2

Rapid Import & Export of Data Files

fcs


Applications Overview

NovoCyte’s expandable laser capabilities along with configurable optical detection design guarantee scientists the ability to advance research and to explore new applications in various areas of cellular analysis. NovoCyte provides a user friendly software interface enabling fast data acquisition and analysis of various flow cytometric applications.

Apoptosis

Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments

Cell Cycle Analysis

Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. With the NovoCyte flow cytometer, detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction.

 

Cell Cycle Analysis Figure

Cell Signaling

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Immunophenotyping

Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. The ACEA NovoCyte flow cytometer can be used for multi-parameter lymphocyte sub-population counting and phenotyping, helper T lymphocyte differentiation and functional study, new sub-population identification, disease specific sub-population identification, and cell surface marker detection.

immunophenotyping

Intracellular Protein Detection

Flow cytometry has traditionally been used to detect extracellular proteins to identify different cell populations. With the advancement of technology and reagents in recent years, we now are able to easily detect intracellular proteins in individual cells. One very useful application for the detection of intracellular proteins is to measure specific cells signaling pathways. These pathways are often altered in disease states, such as cancer, and are now easily detected using flow cytometry providing single cell analysis of these signaling changes.
percp

Jurkat cells were either left in culture(pERK unstim) or stimulated with cell stimulation cocktail for 1hr (pERK +stim).  Cells were washed, fixed and permeablized, followed by staining with anti-phospo-ERK1/2 (T204/Y202) PerCP antibody for 1hr.  Cells were washed and analyzed on the NovoCyte flow cytometer for phospho-ERK levels and compared using a histogram overlay generated with the NovoExpress acquisition and analysis software.

Proliferation

Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

 

App Note 6 Image

Spec Sheets

NovoCyte QC Particles Lot File

Introduction:

1. Download the QC particles lot file corresponding to the lot number of the QC particles.
2. Save the QC particles lot file to the “\QC\QC Beads” folder in the NovoExpress installation directory ( the default installation directory for NovoExpressTM is C:\Program Files (x86)\NovoExpress ).
3. Run NovoCyte QC test.

Downloads:

Note: Please upgrade NovoExpress to 1.0.2 or above version to use the QC particles lot files.(Right click on the corresponding lot number, choose " save link as ", and save to local)

Videos: Product Overviews

Instrument – Includes Workstation & NovoExpress Software

Model Laser Configuration Description Cat. No.
ACEA NovoCyte® 1000 Blue Laser (488 nm) 3 color, FSC, SSC 2010001
ACEA NovoCyte® 2000 Blue / Red Laser (488/640 nm) 4 color, FSC, SSC 2010002
ACEA NovoCyte® 2060 Blue / Red Laser (488/640 nm) 6 color, FSC, SSC 2010004
ACEA NovoCyte® 3000 Blue / Red / Violet Laser (488/640/405 nm) 13 color, FSC, SSC 2010011
ACEA NovoCyte® 3000RYB Red / Yellow / Blue Laser (640/561/488 nm) 13 color, FSC, SSC 2010041
ACEA NovoCyte® 3000VYB Violet / Yellow / Blue Laser (405/561/488 nm) 13 color, FSC, SSC 2010042
ACEA NovoCyte® 2100Y Yellow / Blue Laser (561/488 nm) 10 color, FSC, SSC 2010043
ACEA NovoCyte® 1000 and NovoSampler® Pro Combo Blue Laser (488 nm) 3 color, FSC, SSC 2010047
ACEA NovoCyte® 2000 and NovoSampler® Pro Combo Blue / Red Laser (488/640 nm) 4 color, FSC, SSC 2010048
ACEA NovoCyte® 2060 and NovoSampler® Pro Combo Blue / Red Laser (488/640 nm) 6 color, FSC, SSC 2010049
ACEA NovoCyte® 3000 and NovoSampler® Pro Combo Blue / Red / Violet Laser (488/640/405 nm) 13 color, FSC, SSC 2010050
ACEA NovoCyte® 3000RYB and NovoSampler® Pro Combo Red / Yellow / Blue Laser (640/561/488 nm) 13 color, FSC, SSC 2010044
ACEA NovoCyte® 3000VYB and NovoSampler® Pro Combo Violet / Yellow / Blue Laser (405/561/488 nm) 13 color, FSC, SSC 2010045
ACEA NovoCyte® 2100Y and NovoSampler® Pro Combo Yellow / Blue Laser (561/488 nm) 10 color, FSC, SSC 2010046

Accessories, Reagents and Kits

Description Cat. No.
ACEA NovoSampler® Pro 2020073
NovoCyte® SIP Upgrade Kit 2020083
System Upgrade from NovoCyte® 1000 to 2000 2020039
System Upgrade from NovoCyte® 1000 to 2060R 2020040
System Upgrade from NovoCyte® 2000 to 2060R 2020041
System Upgrade from NovoCyte® 2000 to 3000 2020084
System Upgrade from NovoCyte® 2060R to 3000 2020042
System Upgrade from NovoCyte® 1000 to 3000 2020043
System Upgrade from NovoCyte® 1000 to 2100Y 2020074
System Upgrade from NovoCyte® 2000 to 3000RYB 2020075
System Upgrade from NovoCyte® 2060 to 3000RYB 2020076
System Upgrade from NovoCyte® 2100Y to 3000VYB 2020077
High Performance Computer Laptop Upgrade 2020084
High Performance Computer Desktop Upgrade 2020085
ACEA NovoExpress® Software, Single License 1010011
ACEA NovoExpress® Software, Site License 1010017
1-Year Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030006
6-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030008
2-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 1 2030009
1-Year Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030012
6-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030013
2-Month Maintenance Kit for NovoCyte® Flow Cytometer Version 2 2030014
NovoCyte® QC Particles (2mL; 50 tests) 8000001
Sphero™ 8 Peaks Rainbow Calibration Particles 8000002
Sphero™ 1 Peak Ultra Rainbow Fluorescent Particles 8000003
NovoCyte® Flow Cytometer Starter Kit 8100002
NovoFlow® Sheath Fluid (1X, 10L) 875B601
NovoFlow® Sheath Fluid (100X, 20mL) Box of 10 bottles (20mL/bottle) 871B607
NovoClean™ Solution (1X, 500mL) 872B602
NovoClean™ Solution (5X; 500mL) 872B609
NovoClean™ Solution (5X; 100mL) 871B609
NovoRinse® Solution (1X; 500mL) 872B603
NovoRinse® Solution (5X; 500mL) 872B610
NovoRinse® Solution (5X; 100mL) 871B610

Extended Warranties – Service Contract Options

Note: Each year of extended service warranty includes 1-year Maintenance Kit Catalog No. 2030006

Description Cat. No.
1-Year Extended Service Warranty for NovoCyte® One-Laser System 2040003
2-Year Extended Service Warranty for NovoCyte® One-Laser System 2040004
3-Year extended Service warranty for NovoCyte® One Laser System 2040016
4-Year extended Service warranty for NovoCyte® One Laser System 2040017
5-Year extended Service warranty for NovoCyte® One Laser System 2040018
6-Year extended Service warranty for NovoCyte® One Laser System 2040019
7-Year extended Service warranty for NovoCyte® One Laser System 2040020
1-Year extended Service warranty for NovoCyte® Two Lasers System 2040005
2-Year extended Service warranty for NovoCyte® Two Lasers System 2040006
3-Year extended Service warranty for NovoCyte® Two Lasers System 2040021
4-Year extended Service warranty for NovoCyte® Two Lasers System 2040022
5-Year extended Service warranty for NovoCyte® Two Lasers System 2040023
6-Year extended Service warranty for NovoCyte® Two Lasers System 2040024
7-Year extended Service warranty for NovoCyte® Two Lasers System 2040025
1-Year extended Service warranty for NovoCyte® Three Lasers System 2040007
2-Year extended Service warranty for NovoCyte® Three Lasers System 2040008
3-Year extended Service warranty for NovoCyte® Three Lasers System 2040026
4-Year extended Service warranty for NovoCyte® Three Lasers System 2040027
5-Year extended Service warranty for NovoCyte® Three Lasers System 2040028
6-Year extended Service warranty for NovoCyte® Three Lasers System 2040029
7-Year extended Service warranty for NovoCyte® Three Lasers System 2040030
Preventative Maintenance Service Warranty (includes Parts and Labor for 6 Months & 1 Year PMs Only) 2040015

Replacement Parts and Accessories

Description Cat. No.
Spigot for NovoFlow® Sheath Fluid Refill 2020002
NovoCyte® Fluidics Station 2020003
NovoFlow® Sheath Fluid Container (3L) 2020004
Waste Container (3L) 2020005
NovoRinse® Solution Container (500mL) 2020006
NovoClean™ Solution Container (500mL) 2020007
Fluid Container Quick Coupler and Tubing Set 2020008
12 x 75 mm Tube Holder 2020010
12 x 75 mm Tube Holder Version B 2020068
1.5 mL Microfuge Tube Holder 2020011
Eppendorf Tube Holder Version B 2020069
NovoSampler® 24 Tube Rack 2020014
NovoSampler® Connection Cable 2020038
NovoCyte® Workstation (NovoExpress® Pre-installed) 2020015
NovoCyte® Workstation Monitor 2020016
NovoCyte® Instrument Power Adapter 2020017
NovoCyte® Instrument Power Cord Set 2020044
USB Cable (NovoCyte® Instrument to Workstation) 2020021
DVI Cable (NovoCyte® Instrument to Fluidics Station) 2020022
NovoCyte Decontamination Kit Version 1 2030015
NovoCyte Decontamination Kit Version 2 2030016
NovoCyte® Fluidics Upgrade (V1 to V2) Kit 2020080