Receptor Signaling: Nuclear Hormone-Mediated Signaling

ACEA Biosciences xCELLigence

Estrogen Specific Response Monitored on xCELLigence System

Application_Book_07_01
The estrogen receptor agonist 17β-estradiol (E2) induced a unique kinetic response profile with delayed cell index growth in the human breast cancer cell line T-47D. Such response was abolished by the estrogen receptor antagonist ICI182780 (A). The progesterone antagonist mifepristone cannot abolish E2 response (B), but rather had a synergistic effect with E2 on T-47D cells. (Data and figures adapted from Jin C, et. al., 2011).

Progesterone Specific Response Monitored on xCELLigence System

Application_Book_07_02
Progesterone induced unique kinetic response profile with a biphasic cell index curve in the human breast cancer cell line T-47D. This response was blocked by the progesterone antagonist mifepristone (A), but could not be abolished by the estrogen receptor antagonist ICI182780 (B). (Data and figures adapted from Jin C, et. al., 2011).

Key Benefits 
  1. Label-free nature allows for sensitive detection of endogenous receptor activity.
  2. Kinetic response profiles may be diagnostic for specific pathways.
  3. Ability to differentiate cytotoxicity and proliferation with a single experiment.
  4. Real time data can identify the optimal time to monitor different ligand effects with standard assay.
Nuclear Hormone Receptor Supporting Information:

  • Nuclear Hormone Receptor Publications 
  1. Real-Time Growth Kinetics Measuring Hormone Mimicry for ToxCast Chemicals in T-47D Human Ductal Carcinoma Cell. Daniel M. Rotroff, David J. Dix, Keith A. Houck, Robert J. Kavlock, Thomas B. Knudsen,Matthew T. Martin, David M. Reif, Ann M. Richard, Nisha S. Sipes, Yama A. Abassi, Can Jin, Melinda Stampfl, and Richard S. Judson. Chem. Res. Toxicol. 2013;26(7):1097–1107.
  2. The Aryl Hydrocarbon Receptor Mediates Leflunomide- Induced Growth Inhibition of Melanoma Cells. Edmond F. O’Donnell, Prasad Rao Kopparapu, Daniel C. Koch, Hyo Sang Jang, Jessica Lynne Phillips, Robert L. Tanguay, Nancy I. Kerkvliet, Siva Kumar Kollur.
    PLoS One. 2012;7(7):e40926.
  3. A Novel Sensitive and Selective Real-time Cellular Assay for Detection of Endocrine Disruptors Using Native Endocrine Signaling Pathways. Jin, C., Abassi, Y., Xu, X. & Wang X. Endocr Rev. 2001;Vol. 32 (03_MeetingAbstracts):1-107.